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American Society of Brewing ChemistsIn the LabSampling PlanToolsHemocytometer

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Hemocytometer

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Tools Used in the Sampling Process

Specific tools are needed to analyze the samples taken during the sampling process. Select one of the tools below to learn more about it, including when and where it is used during the Sampling Process.


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The Hemocytometer ​​

A hemocytometer is a small, specialized device designed for use with a microscope to quantitatively determine the number of individual cells in a small amount of fluid. A standard hemocytometer is the size of a large microscope slide, made of glass, and contains a central chamber where the fluid to be examined is inserted. When the hemocytometer is viewed under a standard microscope, the visual plane contains clear gridded areas. By counting the number of cells in this gridded area, one can quantify the number of cells in a volume of fluid, and thereby calculate the cellular concentration of a larger amount of fluid through multiplication. In the context of brewing, a hemocytometer is used to accurately ascertain the yeast concentration in a slurry or extract. In a standard brewing sampling plan, a hemocytometer is used in concert with a microscope to determine yeast concentration when preparing the yeast, as well as during fermentation.


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