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A-30: The use of electron paramagnetic resonance (EPR) technology for advancing sensory beer flavor stability predictions and brewery improvements

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L. MARQUES (1), M. H. Espinosa (1), R. T. Foster (2), W. Andrews (1); (1) Molson Coors Canada, Toronto, ON, Canada; (2) MillerCoors LLC, Golden, CO, U.S.A.

Sensory II
Thursday, June 5 - 10:00 a.m.-11:45 a.m.
Lobby Level, Empire Ballroom

To standardize a method for the forced sensory aging of several types of lager and ale beers, an attempt was made to correlate electron paramagnetic resonance (EPR) results with sensory data on samples subjected to various time–temperature treatments. Forced aging methods used were 3 days at 27°C and 1 day at 40°C, both of which have been used to represent a one month normal shelf life (1 month at 20°C). Standard EPR “area” metric and sensory analyses were completed on three types of forced aged beer samples: standard lager, low alcohol lager, and standard ale. Trained sensory panelists evaluated these packaged beer samples following standard descriptive methods and rated a total of 57 attributes, of which 4 attributes related to staling. The four staling attributes (oxidized, papery, leathery, and catty) were combined for data analysis. For all three types of beer, a significant relationship between EPR area and sensory oxidation scores was found at 40°C for 1 day. Conversely, sensory and EPR area results for samples incubated at 27°C for 3 days did not have a significant relationship. Further, using the new EPR area metric, which represents the amount of free radicals present in the sample, brewing changes were made to lower the EPR area values in five breweries over three years. Mitigation of trace metals and lowering dissolved oxygen while increasing SO2 slightly generally resulted in a reduction in EPR area values. This plan resulted in a dynamic potential flavor stability improvement program that inculcated longer beer shelf life into packaged beer during this baseline period.

Laura Marques received a B.S. degree in chemistry from the University of Western Ontario and diploma in science laboratory technology from Fanshawe College in London, ON, Canada. She began employment at Diagnostix Inc. in 2001 as an R&D chemist working on an effective mycotoxin regimen used in the national grain inspection industry. Since 2006, she has worked for Molson Coors Canada in the role of chemist at the central laboratory. In addition to serving on two ASBC subcommittees she has been published in the Journal of Soil Sciences for work using yeast estrogen screen assays.

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