A-15: Development of ultra-low–gluten barley for brewing and food applications for subjects with gluten intolerance, or coeliac disease

C. HOWITT (1), M. Blundell (1), G. Tanner (1); (1) CSIRO, Canberra, Australia

Regulations and Guidelines
Wednesday, June 4 - 1:00 p.m.-2:45 p.m.
Level 4, Red Lacquer Ballroom

Within populations worldwide there are two groups of people who avoid gluten: those who suffer from coeliac disease and those who have a non-coeliac gluten intolerance. Coeliac disease is well characterized, but the basis for non-coeliac gluten intolerance remains uncharacterized. Both groups require a lifelong dietary exclusion of gluten proteins from wheat (gliadin and glutenins), barley (hordeins), rye (secalins), and in some individuals oats (avenins). This often leads to the consumption of a diet that is low in fiber, high in fat, and more expensive. To address these issues we have identified barley lines that are deficient in different classes of hordeins (gluten) and, through a crossing program, have developed a new type of barley, ultra-low gluten (ULG), that can be considered gluten-free in many jurisdictions, as the gluten content is below 20 ppm. Initial brewing trials have shown that, despite some limitations due to the smaller grain size, the grain could be malted under standard conditions and acceptable beers can be produced from the malt. In order to increase the seed size we have crossed the triple null lines into Australian commercial malting barleys varieties, identified hulled, hordein triple-null lines in three backgrounds (cvs. Sloop, Yagan, and Baudin), and intercrossed these lines to produce 12,000 bi-parental lines. The best of these had improved seed weight and agronomics near those of commercial malting barley. We have also developed a hull-less version of the grain for food applications. Here we report on the development of the grain and the results of the initial malting and brewing trials, which are the first steps toward producing a gluten-free barley-based beer.

Crispin Howitt received a Ph.D. degree from the Australian National University in 1995. His first post-doctoral fellowship was at Arizona State University. In 2000 he moved to CSIRO, Canberra, Australia, to work on starch properties and grain quality. Since 2009 he has led CSIRO’s research into grain quality.

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