Analytical Session
Cheryl G Ermey, John I. Haas, Inc., Yakima, WA USA
Co-author(s): Joyce Carr, John I. Haas, Inc., Yakima, WA, USA
ABSTRACT: A modified rapid method has been developed for
the analysis of hop essential oils by GC-FID based on rapid
characterization of hop essential oils using gas
chromatography–time-of-flight mass spectrometry. This method, which was
published in the Journal of the American Society of Brewing Chemists
(60(3):116, 2002), is able to successfully identify 40 key common
essential oils. The author’s modification, which utilizes GC-FID,
provides a rapid method for the identification of the four primary hop
essential oils: myrcene, humulene, caryophyllene, and farnesene. The
current ASBC method for GC analysis of hop oils results in a run time of
86 min. The fast GC method utilizes a smaller and more efficient DB-5
column with a run time of less than 9 min, resulting in a decreased GC
analysis time of nearly 10-fold. This modified method works very well
for the primary hops oils myrcene, humulene, caryophyllene, and
farnesene. However, because of the dilute nature of the sample in the
fast GC method, essential oils that occur at less than 1% (e.g.,
geraniol, linalool) may not be reliably quantitated. An adjustment in
the sample dilution protocol may make it possible to include these
lesser compounds.
Cheryl Ermey has a bachelor’s degree in
biology, with a minor in chemistry, from Central Washington University
in Ellensburg, WA. She has been employed by John I. Haas, Inc. in the
Quality Assurance laboratory since 2008. Prior to working at Haas,
Cheryl worked as a microbiology technician for Ag Health Labs in
Sunnyside, WA, and as a chemistry technician for Battelle Toxicology
Northwest in Richland, WA. She has several years of experience working
with gas chromatography, both at Haas and at Battelle. Cheryl also spent
five years as a volunteer firefighter with the West Valley Fire
Department in Yakima County.
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