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A methodology utilizing HPLC was suggested and validated for determination of the glycerol in beer. The chromatographic separation was carried out on a Waters Sugar-Pak1 (6.5 mm i.d. by 300 mm in length) cations ion exchange column with EDTA calcium disodium salt solution at 0.05 g L–1 as mobile phase and a flow rate of 0.5 mL min–1. Beer (20 µL) filtered by a 0.22-µm membrane syringe filter was directly injected onto the HPLC system for analysis. The validation parameters included linearity, precision, limits of detection and quantitation, and accuracy. The calibration curve was linear (R2 = 0.998) within the calibration range. The precision and recovery was 1.1 and 98.5%, respectively. The limit of detection and limit of quantitation for the glycerol were 2.2 and 7.3 µg/L, respectively. This method was applied to quantify the glycerol in 34 beer samples purchased from the marketplaces in China. The analysis results showed that the glycerol content of the beers varied from 925.2 to 1,502.74 mg/L. Keywords: Beer, Glycerol, HPLC
