PATRICK S. JENSEN (1), Scott W. Garden (1)
(1) John I. Haas, Inc., Yakima, WA
Ultra-high-performance liquid chromatography (UHPLC) technology has
the potential to decrease the time associated with hop acid analyses and
reduce the amount of solvent necessary per analysis, all without
compromising chromatographic resolution. UHPLC technology is able to
improve chromatographic efficiency and performance by accommodating the
high system back pressures associated with the use of high-resolution
columns prepared with less than two-micron packing. During this study,
hop samples were analyzed using industry accepted HPLC conditions (ASBC
Hops-14 and Hops-16) as well as by a method developed for an UHPLC/PDA
system (Agilent 1290 UHPLC). The hop samples examined were selected from
a range of hop products (cone hops, pellets, extracts) containing
α-acids, β-acids, and/or iso-α-acids. No significant differences in
α-acid or iso-α-acid analysis were observed when results from HPLC and
UHPLC methods were compared. β-Acid results varied between
chromatographic methods depending on the type of hop sample analyzed.
The results of this study indicate that further work on UHPLC method
development for routine hop analysis is justified and should be
explored.
Patrick Jensen received a B.S. degree in chemistry with a
specialization in biochemistry from Central Washington University in
Ellensburg. After graduation in 2004, he began his employment in the hop
industry working in quality assurance laboratories. Since 2009 he has
been a laboratory supervisor for John I. Haas Inc. and is responsible
for the analytical analysis of hop products. He also teaches analytical
and introductory chemistry courses at Heritage University in Toppenish,
WA.
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