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American Society of Brewing ChemistsEventsMeeting Archives2011 Meeting

Display Title
A novel homogeneous enzyme immunoassay for rapid on-site analysis of deoxynivalenol (DON) in grain

Page Content
SHERMAN H. CHAN (1)
(1) ThermoFisher Scientific, Clinical Diagnostics Division

A new on-site test for deoxynivalenol (DON) was developed using a novel homogeneous enzyme immunoassay (HEIA) method. The test mechanism is described in detail and data is presented comparing the test’s performance with commercial ELISA kits using grain samples (barley, malted barley, and wheat) naturally contaminated with DON at levels determined by HPLC and GC-MS analysis. The accuracy and precision of the DON HEIA test was found to be consistently equivalent to the corresponding ELISA systems. These results indicate that the new HEIA test can be effectively used for on-site DON testing and offers additional advantages of speed and simplicity.

Sherman Chan received his bachelor’s degree from the National Taiwan University and master’s degree in cereal science and technology from North Dakota State University, Fargo. Sherman was employed at the Fleischmann Malting Company as quality control lab manager and then at the Pabst Brewing Company as a principal research chemist. Sherman joined Rahr Malting Company in 1980 as the technical director in charge of research and quality control until his retirement in 2006. He has been short course instructor for ASBC and MBAA and has worked as a malting consultant and short course instructor for the U.S. Grains Council to promote U.S. barley in Asia and South America. He has been a member of ASBC, MBAA, the AMBA Technical Committee, and IoB. He has served ASBC on the local and national levels. In addition to serving on technical subcommittees and the Technical Committee, he has served as president-elect, president, and past president of ASBC. During the past few years, he has worked as a technical consultant for the ThermoFisher Scientific Clinical Diagnostics Division.

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