VIEW ARTICLE    DOI: 10.1094/ASBCJ-52-0012

Biochemical and Genetic Characterization of a 2-Deoxy-d-glucose Resistant Mutant of Saccharomyces cerevisiae. Tony D'Amore and Terrance M. Dowhanick, Brewing Research Department, Labatt Breweries of Canada, 150 Simcoe Street, London, Ontario, Canada N6A 4M3. J. Am. Soc. Brew. Chem. 52:0012, 1994.

Biochemical and genetic characteristics of a 2-deoxy-d-glucose resistant mutant of Saccharomyces cerevisiae were examined. Fermentation studies indicated that the mutant possessed greater maltose uptake ability in the presence of glucose than did the parental strain. In addition, growth of cells on glucose and/or maltose resulted in differences in sugar transport and growth rates between the mutant and the parental strains. Gene expression monitored by³⁵ S-methionine in vivo labeling of nascent solubilized cellular proteins under the various growth conditions revealed alterations in protein profile. Among the radiolabeled polypeptides, maltose-inducible and glucose-repressible products were observed. The identified proteins may play a role in the repression/derepression mechanism in yeast.

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