VIEW ARTICLE    DOI: 10.1094/ASBCJ-47-0109

Electrophoretic Banding Patterns of Chromosomal DNA from Yeasts. Yoshihiro Takata, Junji Watari, Norio Nishikawa, and Kôzô Kamada, Research & Development Laboratories, Sapporo Breweries Ltd., Okatohme 10, Yaizu-shi, Shizuoka, 425 Japan. J. Am. Soc. Brew. Chem. 47:0109, 1989.

The electrophoretic banding patterns of chromosomal DNA from various yeasts were investigated using OFAGE (orthogonal-field-alternation gel electrophoresis). Yeasts belonging to Saccharomyces cerevisiae, including brewer's strains, showed more than 11 chromosomal DNA bands ranging from 250 to 1,000 kb or more. Most strains of the genera Kluyveromyces, Schwanniomyces, Schizosaccharomyces, Zygosaccharomyces, and Candida revealed several bands all larger than 1,000 kb. When cell fusants between a brewer's strain and a killer strain (Saccharomyces cerevisiae) were examined, their banding patterns clearly differed from those of the parents; the mixture of chromosomes derived from the parents was confirmed by Southern hybridization. OFAGE was found to be an effective tool to aid genetic research on a wide range of yeasts and yeast cell fusants.

Keywords: Chromosomal DNA, OFAGE, Protoplast fusion, Yeast