VIEW ARTICLE    DOI: 10.1094/ASBCJ-46-0037

A Review of Quick, Reliable, and Simple Check Methods for Barley and Malt Based on the Carlsberg Seed Fixation System. Sten Aastrup, Carlsberg Research Center, Gamle Carlsberg Vej 10, DK-2500 Copenhagen, Valby, Denmark. J. Am. Soc. Brew. Chem. 46:0037, 1988.

A series of new methods of analysis that all use the same basic system, the "Carlsberg Seed Fixation System," was developed in an attempt to meet the needs of the brewing industry for rapid, reliable, and simple methods for controlling the quality of barley and malt. The European Brewery Convention has approved the methods for pregermination in barley and determination of malt modification and homogeneity. For barley analyses, the method for pregermination based on fluorescein dibutyrate staining is described, and a comparison of results obtained by this method and by traditional determinations of germinative energy is presented. In addition, methods for distinguishing between proanthocyanidin-free barleys and normal barleys, for distinguishing between dead and living kernels, and for the selection of high-lysine barleys are described. For malt analyses, the method for malt modification and homogeneity based on Calcofluor staining is described. This method is shown to be applicable in research and plant breeding as well as in evaluation of final malt. A comparison between results obtained using the Calcofluor staining method and the Friabilimeter method is presented.

Keywords: Homogeneity, Malt modification, Pregermination, Proanthocyanidins, Seed fixation, Viability