VIEW ARTICLE    DOI: 10.1094/ASBCJ-44-0098

The Genetic Modification of Brewing Yeast with Recombinant DNA. Edward Hinchliffe and Christine J. Daubney, Research Department, Bass Public Limited Company, Burton-on-Trent, England. J. Am. Soc. Brew. Chem. 44:0098, 1986.

The presence of 2-µm plasmid DNA was demonstrated in each of seven different strains of brewing yeast investigated. These yeasts were used as recipients for 2-µm-based recombinant DNA plasmids carrying the copper resistance gene of Saccharomyces cerevisiae and the β-glucanase gene of Bacillus subtilis. When grown under nonselective brewery conditions, the transformed copper-resistant phenotype of brewing yeast was shown to be relatively stable. The brewing properties of yeast harboring recombinant DNA plasmids were indistinguishable from their unmodified parental counterparts.

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