VIEW ARTICLE    DOI: 10.1094/ASBCJ-42-0164

Breeding of a Brewer's Yeast Possessing Anticontaminant Properties. T. Sasaki, J. Watari, M. Kohgo, N. Nishikawa, and Y. Matsui, Research and Development Laboratories, Sapporo Breweries Ltd., Okatohane, Yaizu 425, Japan. J. Am. Soc. Brew. Chem. 42:0164, 1984.

Antibacterial and anti-wild yeast characters were introduced into a brewer's yeast to achieve a more positive microbial assurance in brewing. An antibacterial yeast that inhibits the growth of 11 beer-spoilage bacteria was screened from our stock cultures and mated with a killer yeast active against many brewery wild yeasts. After sporulation of the hybrid, a haploid clone (AGK27) possessing the two anticontaminant properties was obtained. This clone was protoplast-fused with our brewery yeast, and one fusant, MAK61, was selected out of many hybrid products. MAK61 had both anti-wild yeast and antibacterial characters, and the beer produced was considered acceptable, although the fermentation rate was a little slower than that of our current brewery yeast. This work demonstrates the practical breeding of a brewer's yeast using protoplast fusion to improve brewing characteristics.

Keywords: Antibacterial, Breeding, Brewing, Killer yeast, Protoplast fusion, Yeast