VIEW ARTICLE    DOI: 10.1094/ASBCJ-41-0036

Fast High Performance Liquid Chromatography Analysis of Hop Bitter Compounds. M. Verzele, C. Dewaele, and M. Van Kerrebroeck, Laboratory of Organic Chemistry, State University of Ghent, Krijgslaan, 281 (S. 4), B-9000 Gent, Belgium; and J. Strating and L. Verhagen, Technical Center of Heineken Breweries, Zoeterwoude, the Netherlands. J. Am. Soc. Brew. Chem. 41:0036, 1983.

Fast high performance liquid chromatography (HPLC) analysis of iso-α-acids and of α- and β-acids was performed. Sample preparation was reduced to a minimum. The time of the HPLC run for beer and hops or hop extract analysis was reduced to 5 min. 2,6-Di-tertiary butylphenol was used as an internal standard for iso-α-acids analysis. The ?-nitroanilide of myristic acid was used as an internal standard for α-acids analysis.

Keywords: α-Acids, β-Acids, Beer, Hops, Hop extracts, Humulone, Iso-α-acids, Lupulone