VIEW ARTICLE    DOI: 10.1094/ASBCJ-40-0141

Application of High Performance Liquid Chromatography in Brewing. I. Determination of Carbohydrates and Alcohol. M. Dadic and G. Belleau, Molson Breweries of Canada Ltd., Montreal, Quebec H2L 2R5. J. Am. Soc. Brew. Chem. 40:0141, 1982.

Aminex® HPX-87, Dextropak (alone or in combination) and Aminex HPX-42 were used with pure water as an isocratic eluent. An alcohol-precipitated dextrin was used as calibration standard for determination of nonfermentable carbohydrates. Individual oligosaccharides (DP 4 to DP 13) were also measured. Fermentable sugars were expressed as maltotriose (trisaccharides), maltose (disaccharides), glucose (monosaccharides except fructose), and fructose. Noncarbohydrate material was determined from the total carbohydrate content and the specific gravity of the analyzed substrate. Aqueous extracts of a 2- and 6-row barley, as well as the corresponding malts, were analyzed. The effect of mashing time and temperature on the carbohydrate profiles of the sweet worts was discussed. Carbohydrates in a torrefied wheat and in hop pellets were analyzed after aqueous extraction. Carbohydrate profiles of two different corn syrups were compared. Chromatography on Aminex HPX-87 column was used to monitor wort fermentation and utilization of fermentable sugars by yeast. Ethanol and glycerol were measured simultaneously with carbohydrates in ale and lager. The optimal chromatographic conditions were recommended for the analysis of a particular brewing substrate. High performance liquid chromatography of carbohydrates could be used for monitoring barley germination.

Keywords: Aminex® HPX-87, Aminex® HPX-42, Beer, Brewing materials, Dextropak, Wort