VIEW ARTICLE    DOI: 10.1094/ASBCJ-40-0078

NOTE A New Method for Ethanol Measurement Utilizing an Immobilized Enzyme. Marc Mason, Yellow Springs Instrument Co., Yellow Springs, OH 45387. J. Am. Soc. Brew. Chem. 40:0078, 1982.

Ethanol in 10 different beer samples was determined, comparing an immobilized enzyme system with the AOAC distillation/pycnometer procedure. In the enzymatic system, alcohol oxidase is immobilized in a thin microporous membrane. This membrane is mounted on an amperometric electrode. When ethanol enters the membrane, it is oxidized, producing H₂O₂ and acetaldehyde. The H₂O₂ is measured at the electrode. Precision for the enzymatic method was excellent, ±0.05% (v/v) ethanol. Agreement with the AOAC procedure was good.

Keywords: Alcohol oxidase, Beer, Ethanol, Immobilized enzyme