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Nitrogen analysis in beer using a Zahm Custom Sample Bottle.
Gregory C. Kreder. Brewing Technical Services, Anheuser-Busch Inc., #1 Busch Place, St. Louis, Missouri 63118.

Nitrogenated draft beer has been available for years and provides the consumer with an alternative to traditional draft beers. Accurate analysis of the nitrogen content in beer is needed to ensure the quality and consistency of the product. While both in-line and portable nitrogen meters are available, they are expensive. Furthermore, the use of a dedicated nitrogen analyzer adds another instrument to be maintained and calibrated. The use of Orbisphere packaged beer analyzers has become standard in many breweries for measurement of Total Package Oxygen (TPO) and CO2. These instruments can also calculate nitrogen based on headspace partial pressure. The use of a laboratory instrument offers certain advantages in that they are more easily maintained and calibrated than in-line instruments. However, the accurate measurement of nitrogen in beer intended for draft dispense with a laboratory instrument requires the presentation of a representative sample. This does present certain difficulties due to in insolubility of nitrogen in beer and the requirement of a headspace component in the sample. A solution to this problem was provided with the development of a modified Zahm bottle, fitted with a piston release mechanism to create package headspace. The newly developed nitrogen measurement procedure was evaluated for reproducibility and was compared with the results obtained using conventional analysis for non-CO2 gas.

Gregory Kreder received a B.S. in Chemistry from Southern Illinois University in Edwardsville. He began employment with Anheuser-Busch in May 1989 as a chemist in the analytical laboratory of Brewing Technical Services. Since January 1990, he has been conducting research in all facets of brewing, including new product development in the Brewing Technical Services Pilot Brewery. He has served the ASBC on the local level as secretary through Past President (1996-2000).

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