P-1

The influence of raw hop storage condition on the quality of hops and beer.
TSUNEHIRO IKEDA (1), Toru Kishimoto (1), Atsushi Suzuki (1), Tomoki Nanba (2), Nobuo Kaji (1), Yutaka Miyamoto (1), Tomomichi Oga (1), Kazunori Shibata (1), and Katsuyuki Kawatsura (1). (1) Asahi Breweries, Ltd., Moriya, Ibaraki, Japan, and (2) Asahibeer Malt, Ltd.

From the brewer's standpoint, the most important hop components consist of bitter substances, oils, and polyphenols. In Europe, there was no cold storage warehouse for raw hops after harvesting until quite recently. Therefore we were afraid of changes in the quality of raw hops during storage. The constituents of hops are affected by oxygen, temperature, storage time, and humidity. It has been reported that cold storage of hops avoids the reduction in alpha acids normally associated with room temperature storage. We conducted the storage test of raw hops under laboratory and normal warehouse conditions to examine the effect of storage temperature on hops and beer. In the laboratory tests, we evaluated the common indexes used to determine hops and beer deterioration and decided whether we could use them. In the normal warehouse tests, we examined the changes in quality under actual conditions to determine the effectiveness of using the above indexes. In the laboratory, we used the Magnum, the Hersbrucker, and the Saaz; the hops were stored at 4, 15, and 30°C for 5 months. We then examined the changes in hops and beer quality with deterioration indexes. Regarding deterioration indexes for hops, we determined alpha acid content using the HPLC method and LCV, the ratio of the former to the latter (HPLC/LCV ratio), the Hop Storage Index (HSI), and Hop Deterioration Index (HDI). Consequently with increasing temperature, we found a wider difference in the indexes. Therefore the storage temperature affected hops quality, and the foregoing methods were adopted as deterioration indexes for hops. We brewed with the stored hops in 200-L pilot plants, and examined the effect on beer quality. As to deterioration indexes for beer, we evaluated the ratio of iso-alpha-acid content to bitter units, polyphenols, anthocyanogen, and low molecular polyphenols by the HPLC method. Also, beer quality was evaluated by sensory analysis. We found changes in the indexes for taste and aroma. The storage temperature affected the beer quality, and we adopted the foregoing methods as deterioration indexes for beer. In the normal warehouse tests, Japanese Shinshu-Wase, were used and stored at 4°C or normal temperature for 5 months. We obtained almost the same results as in laboratory tests, indicating that the storage temperature affected the hops and beer quality under actual conditions. We should determine whether to use cold storage warehouse or normal warehouse from the viewpoint of costs and quality.

Tsunehiro Ikeda received a Master of Agricultural Chemistry in Kyoto Prefectural University. In April 1988, he began to work at Non-Alcholic Beverage and Foodstuffs Research Laboratory as an analyst in Asahi Breweries, LTD. He has studied Bremaster course as a guest student at Weihenstephan University in Germany (1997-1998). In the Hallertau hop cultivation area in Germany he has had a practical training in analysis, cultivation, and production of hops. Since 1998 he has researched hops in the Brewing Research and Development Laboratory, Ibaraki.

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