New Enzymes for Brewing: Promozyme, a Debranching Enzyme and SP-249, a Glucanase. T. Olesen, K. Pommer, and B. Stentebjerg-Olesen, Novo Industri A/S, Bagsvœrd, Denmark. J. Am. Soc. Brew. Chem. 42:0085, 1984.

Two newly developed polysaccharide-degrading enzymes were evaluated. One, a pullulanase, Promozyme™, can be used alone during mashing, wort treatment, or fermentation to increase the fermentability of wort. When used during fermentation in combination with a fungal α-amylase, Fungamyl®, apparent attenuations above 100% can be reached. The other enzyme, a multicomponent carbohydrase, SP-249, degrades the nonstarch polysaccharides of barley and malt, resulting in reduced wort viscosity and improved runoff. SP-249 is more efficient than commercial β-glucanases.

Keywords: Low-carbohydrate beer, Multicarbohydrase, Runoff, Saccharifying enzymes, Wort viscosity