Fast High Performance Liquid Chromatography Analysis of Hop Bitter Compounds. M. Verzele, C. Dewaele, and M. Van Kerrebroeck, Laboratory of Organic Chemistry, State University of Ghent, Krijgslaan, 281 (S. 4), B-9000 Gent, Belgium; and J. Strating and L. Verhagen, Technical Center of Heineken Breweries, Zoeterwoude, the Netherlands. J. Am. Soc. Brew. Chem. 41:0036, 1983.

Fast high performance liquid chromatography (HPLC) analysis of iso-α-acids and of α- and β-acids was performed. Sample preparation was reduced to a minimum. The time of the HPLC run for beer and hops or hop extract analysis was reduced to 5 min. 2,6-Di-tertiary butylphenol was used as an internal standard for iso-α-acids analysis. The ?-nitroanilide of myristic acid was used as an internal standard for α-acids analysis.

Keywords: α-Acids, β-Acids, Beer, Hops, Hop extracts, Humulone, Iso-α-acids, Lupulone