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VIEW ARTICLE DOI: 10.1094/ASBCJ-39-0067
High Performance Liquid Chromatographic Analysis of Beer Bitter Acids. M. Verzele and C. Dewaele, Laboratory of Organic Chemistry, State University of Ghent, Krijgslaan, 271 (S.4), B-9000 Ghent, Belgium. J. Am. Soc. Brew. Chem. 39:0067, 1981.
A quantitative high performance liquid chromatography (HPLC) analysis for beer bitter acids is presented. The method uses 10-µm silica gel modified with octadecyl groups and isocratic elution with tetrabutyl-ammonium hydroxide in acidic methanol-water as ion pair former. Isocohumulones, isohumulones, and isoadhumulones were separated and eluted in that order. β-Phenylchalkone is proposed as an internal standard. Analysis time was less than 15 min.
Keywords: Beer bitter acids, HPLC, Iso-α-acids
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