Analytical Methodology and Determination of N-Nitrosodimethylamine in Beer, Wort, and Malt. K. S. Markl and R. A. Latimer, Anheuser-Busch, Inc., St. Louis, MO 63118. J. Am. Soc. Brew. Chem. 39:0059, 1981.

Analytical methodology for the determination of N-nitrosodimethylamine (NDMA) in beer, wort, and malt was developed. The analysis involved the use of an internal standard, direct extraction of beer or wort with dichloromethane, followed by concentration, and Gas Chromatography-Thermal Energy Analyzer® determination of NDMA. The NDMA content of malt was conveniently calculated from a laboratory wort. Confirmation of NDMA in beer was obtained by chemical ionization mass spectrometry. The methods developed for the measurement of NDMA in beer, wort, and malt were compared with other analytical methods currently in use and, in our studies, they were found to be faster, more accurate, and more repeatable.

Keywords: Analytical method, Beer, Malt, N-Nitrosodimethylamine, N-Nitrosodipropylamine, Wort