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VIEW ARTICLE DOI: 10.1094/ASBCJ-56-0118
Characterization of Hop Acids by Liquid Chromatography with Negative Electrospray Ionization Mass Spectrometry. A. J. P. Hofte and R. A. M. van der Hoeven, Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands; S. Y. Fung and R. Verpoorte, Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Leiden University; and U. R. Tjaden (1) and J. van der Greef, Division of Analytical Chemistry, Leiden/Amsterdam Center for Drug Research, Leiden University. (1) Corresponding author. J. Am. Soc. Brew. Chem. 56(3):118-122, 1998. Accepted August 10, 1998.
The analysis
of hop acids,
humulones and
lupulones, with
negative electrospray
ionization mass
spectrometry
(MS) is described
for two different
chromatographic
systems. One
system focuses
on the complete
resolution of
the ad- and
normal homologues
implying that
no optimal mass
spectrometric
parameters can
be used, whereas
the other system
makes no compromise
to the MS and
yields a high
sensitivity.
The latter system
is especially
suitable for
the fast screening
of bitter acids.
With this system,
the detection
limit with selected
ion monitoring
(SIM)-MS could
be estimated
to be 10 ng/ml
for the humulones
and 5 ng/ml
for the lupulones.
The presence
of hop acids
in beer was
investigated
and was found
to be positive
for humulones,
whereas lupulones
could not be
detected.
Es descrito
el análisis
de ácidos
de lupulo, humulonas
y lupulonas,
por espectrometría
de masas de
ionización
electrorocio
negativo, por
dos sistemas
cromatográficos
diferentes.
Un sistema se
enfoca en la
completa resolución
de los homólogos
normal y ad
implicando que
parámetros
espectrometricos
de masa no optima
pueden ser usados,
mientras que
el otro sistema
no se compromete
al MS y proporciona
una alta sensibilidad.
El ultimo sistema
es especialmente
conveniente
para el rápido
monitoreo de
ácidos
amargos. Con
este sistema
el limite de
la determinación
con espectrometría
de masas por
medio de monitoreo
de ion selective
(SIM) puede
ser estimado
en 10 ng/ml
para las humulonas
y 5 ng/ml para
las lupulonas.
La presencia
de ácidos
de lupulo en
cerveza fue
investigado
y se encontró
era positivo
para humulonas,
mientras que
lupulonas no
pudieron ser
detectadas.
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