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VIEW ARTICLE    DOI: 10.1094/ASBCJ-56-0076

High-Performance Liquid Chromatography Analysis of Tetrahydro-iso-alpha-acids in Beer. Joseph G. Clark (1), Louis J. Burroughs, and James A. Guzinski (2), Kalsec, Inc. P.O. Box 50511, Kalamazoo MI 49005-0511. (1) Current address: Supelco, Supelco Park, Bellefonte PA 16823. (2) Corresponding author. J. Am. Soc. Brew. Chem. 56(2):76-79, 1998. Accepted May 25, 1998.


An improved method for the measurement of tetrahydro-iso-alpha-acids and iso-alpha-acids in beer is presented. The method is based upon the HPLC separation of hop acids on a C8 reverse-phase column using gradient elution with a citrate buffer at a pH of 7.0. The method is suitable for measurement of small additions of tetrahydro-iso-alpha-acids for foam improvement as well as for the determination of trace amounts of iso-alpha-acids in a beer designed to be light stable. Keywords: Beer bitterness, Light-stable beer, Light-struck.


Es presentado un método mejorado para la medición de tetrahidroisoalfa ácidos e isoalfa-ácidos en cerveza. El método esta basado en la separación por HPLC de ácidos de lúpulo sobre columna C8 fase reversa usando gradiente de elución con buffer citrato a pH 7.0. El método permite la medición de pequeñas adiciones de tetrahidroisoalfa ácidos para mejoramiento de la espuma, así como la determinación de cantidades trazas de iso alfa ácidos en una cerveza diseñada a ser estable a la luz.

 
 
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The ASBC Journal publishes scientific papers, review articles, and technical reports dealing with the chemistry and microbiology of brewing ingredients and relevant technology, as well as the analytical techniques used in the malting and brewing industry.