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VIEW ARTICLE DOI: 10.1094/ASBCJ-56-0076
High-Performance
Liquid Chromatography
Analysis of
Tetrahydro-iso-alpha-acids
in Beer. Joseph
G. Clark (1),
Louis J. Burroughs,
and James A.
Guzinski (2),
Kalsec, Inc.
P.O. Box 50511,
Kalamazoo MI
49005-0511.
(1) Current
address: Supelco,
Supelco Park,
Bellefonte PA
16823. (2) Corresponding
author. J. Am.
Soc. Brew. Chem.
56(2):76-79,
1998. Accepted
May 25, 1998.
An improved
method for the
measurement
of tetrahydro-iso-alpha-acids
and iso-alpha-acids
in beer is presented.
The method is
based upon the
HPLC separation
of hop acids
on a C8 reverse-phase
column using
gradient elution
with a citrate
buffer at a
pH of 7.0. The
method is suitable
for measurement
of small additions
of tetrahydro-iso-alpha-acids
for foam improvement
as well as for
the determination
of trace amounts
of iso-alpha-acids
in a beer designed
to be light
stable. Keywords:
Beer bitterness,
Light-stable
beer, Light-struck.
Es presentado
un método
mejorado para
la medición
de tetrahidroisoalfa
ácidos
e isoalfa-ácidos
en cerveza.
El método
esta basado
en la separación
por HPLC de
ácidos
de lúpulo
sobre columna
C8 fase reversa
usando gradiente
de elución
con buffer citrato
a pH 7.0. El
método
permite la medición
de pequeñas
adiciones de
tetrahidroisoalfa
ácidos
para mejoramiento
de la espuma,
así como
la determinación
de cantidades
trazas de iso
alfa ácidos
en una cerveza
diseñada
a ser estable
a la luz. |
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A subscription to the Journal of the American Society of Brewing Chemists is complimentary with membership to the ASBC.
The ASBC Journal publishes scientific papers, review articles, and technical reports dealing with the chemistry and microbiology of brewing ingredients and relevant technology, as well as the analytical techniques used in the malting and brewing industry.
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