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VIEW ARTICLE    DOI: 10.1094/ASBCJ-55-0044

Determination of Inorganic and Organic Anions in Beer and Wort by Capillary Electrophoresis. Tomoyoshi Soga (1), Application Department, Yokogawa Analytical Systems Inc., 2-11-13 Nakacho, Musashino-shi, Tokyo, 180, Japan and Makoto Wakaura, Process Engineering Research and Development Laboratory, Asahi Breweries Ltd., 2-13-1 Ohmorikita, Ohta-ku, Tokyo, 143, Japan. (1) Corresponding author. E-mail: <tomoyoshi_soga@om.jpn.hp.com> Phone: +81-422-52-5645. Fax: +81-422-52-5966. J. Am. Soc. Brew. Chem. 55(2):44-46. Accepted March 31, 1997.

A rapid, easy, and reproducible method for the determination of inorganic and organic anions in beer and wort by capillary electrophoresis was developed. Three inorganic anions (chloride, sulfate, and phosphate) and nine organic acids (oxalate, formate, malate, citrate, succinate, pyruvate, acetate, lactate, and pyroglutamate) were separated and quantified in less than 7 min. Sample preparation was simple and consisted only of degassing the beer by sonication and 1:5 dilution with Milli Q water prior to injection. Satisfactory reproducibility was obtained, and the concentrations of anions were in good agreement with those found by conventional techniques. Keywords: Beer, Capillary electrophoresis, Inorganic anions, Organic acids, Wort.

Un método rápido, fácil, y reproducible para la determinación de aniones orgánicos e inorgánicos en cerveza y mosto por electroforesis capilar fue desarrollado. Tres aniones inorgánicos (cloruro, sulfato, y fosfato) y nueve ácidos orgánicos (oxalato, formato, malato, citrato, succinato, piruvato, acetato, lactato. y piroglutamato) fueron separados y cuantificados en menos de 7 min. Preparación de la muestra fue simple y consistió solamente en desgasificar la cerveza por sonicación y dilución 1:15 con agua Milli Q antes de la inyección. Reproducibilidades satisfactorias fueron obtenidas y las cantidades de aniones tuvieron una buena relación comparada con las técnicas convencionales.

 
 
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The ASBC Journal publishes scientific papers, review articles, and technical reports dealing with the chemistry and microbiology of brewing ingredients and relevant technology, as well as the analytical techniques used in the malting and brewing industry.