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VIEW ARTICLE DOI: 10.1094/ASBCJ-53-0029
Automated Analysis of the Total Polyphenols Content of Beer and Wort Using an Intelligent Autosampler. Shuso Sakuma, Choji Kikuchi, and Masahiro Kowaka, Technical Center, Technology Development Department, Beer Division, Kirin Brewery Co. Ltd, 17-1 Namamugi 1-chome, Tsurumi-ku, Yokohama-shi, 230 Japan. J. Am. Soc. Brew. Chem. 53(1):29-32, 1995. Accepted July 27, 1994.
An automatic analyzer for the determination of the total polyphenol content of beer and wort was developed using a programmable autosampler with a dilutor, an absorbance detector for high-performance liquid chromatography, and an integrator. The sample size was reduced to 1.2 ml, and the concentration of the ammonia reagent used was reduced to 5%. After certain adjustments to the addition and reagent mixing procedures, results almost identical to those of the ASBC method were obtained. The correlation coefficient between this method and the ASBC method was 0.997 for beer samples and 0.995 for wort samples. It required approximately 20 min for one analysis to be completed. A maximum of 30 samples could be analyzed during one cycle. Keywords: Automated analysis, Beer, Total polyphenols, Wort
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A subscription to the Journal of the American Society of Brewing Chemists is complimentary with membership to the ASBC. The ASBC Journal publishes scientific papers, review articles, and technical reports dealing with the chemistry and microbiology of brewing ingredients and relevant technology, as well as the analytical techniques used in the malting and brewing industry. |